Clinical research of NMN and Resveratrol

Margaret Grady
Jul 26, 2023
4 min read

NMN (Nicotinamide Mononucleotide) and resveratrol are two supplements that may work synergistically to promote cellular health, enhance energy production, and support healthy aging.

Together they may slow down cell aging and deliver even more positive effects to the human body. This combination is believed to boost strength, physical endurance, and increase blood flow.

What is NMN?

NMN is one of the main precursors of nicotinamide adenine dinucleotide (NAD+) — an essential enzyme for various critical cell functions, including metabolism, cell growth, and survival. It also plays a key role in boosting energy and stimulating cellular repair.

Studies have shown that NMN could enhance NAD+ biosynthesis and improve disease detection in laboratory mice. For example, in one of the first NMN studies, mice exposed to an NMN supplement experienced increased tissue NMN and NAD+ levels within 15 min in the liver, pancreas, and white adipose tissue.

Long-term effects of NMN supplementation

To determine the effects of long-term NMN administration, scientists conducted a 12-month-long study. Initial findings showed that long-term administration of NMN and dose-dependently significantly suppressed age-associated body weight gain. In addition, mice administered with NMN consumed more food with less weight gain compared to the control group. Interestingly, NMN-administered mice showed enhanced energy metabolism, higher physical activity, and better oxygen consumption.

Furthermore, this clinical research of NMN and Resveratrol shows reverse age-associated gene expression changes, which could induce anti-aging effects at the molecular level. After the conclusion of this research study, researchers performed more than 27 other studies to observe the effects of NMN in mice. These studies confirmed that NMN could:

Improve glucose tolerance and insulin secretion.
Inhibit inflammation, reduce neural cell death.
Reduce the infarct area after ischemia.
Restore physical activity.
Improve learning, memory, and cognitive functions.
Reduced DNA damage.
Improve cardiac functions.

The anti-aging and other benefits of NMN

Researchers also tested the anti-aging effects of NMN and its safety in a double-blind, parallel, randomized controlled clinical trial. This study included 66 healthy subjects between the ages of 40 and 65. They took 300 mg of NMN daily after breakfast for 60 days. In the middle of the study (day 30), NAD+ level showed an increase of 11.3%, whereas the placebo group showed no change. Furthermore, by the end of the study, i.e., day 60, the NAD+ level had increased by 38%.

The study proved that a rise in NAD+ in the NMN consuming group was considerably higher than in the placebo group. Increasing the dose or the duration could have more significance. It also did not cause any specific damaging effects in healthy volunteers.

Researchers administered three dosages (300, 600, and 1200 mg/d) of NMN supplementation to healthy amateur runners during a 6-week exercise training program. The main finding of this study was that NMN supplementation during exercise improved oxygen uptake.

Researchers noticed that combining exercise with NMN supplementation increases oxygen consumption. Additionally, dose-dependent subjects and those with large doses of NMN were better affected, but dosages had no effect on grip strength, the number of push-ups or sit-and-reach reps subjects could complete compared with athletes exercising without supplementation. However, 600 mg/day NMN significantly improved single leg stance test results.

What is Resveratrol and how does it work?

Resveratrol, an antifungal, is a stilbenoid found in a number of plant species. Over 19 model organism-based studies (yeast, nematodes, mice, fruit flies, Mexican fruit flies, and turquoise killifish) revealed that Resveratrol could extend lifespan.

A study demonstrated that Resveratrol could induce autophagy in human cells in vitro and promote longevity through the Sirt1 gene-dependent induction of autophagy — Sirt1–Sirt7 genes have long been key anti-aging genes. In addition, Resveratrol could also promote the activities of nuclear-factor kappa B (NF-κB) and the Sirt1 gene to decrease the levels of inflammatory markers.

The study also showed that Resveratrol has protective effects against neurodegenerative diseases by enhancing the secretion of neurotransmitters, increasing the production of new neurons, and decreasing neuroinflammation and oxidative stress. Other studies show that this supplement has a cardio protective effect by enhancing nitric oxide production, modulating the activity of the renin-angiotensin system, and ameliorating oxidative stress. Many studies have shown that resveratrol is effective in preventing and treating cancers. It does so by significantly inhibiting cancer cell proliferation, migration, and invasion.

Should you take NMN and Resveratrol together?

Clinical research of NMN and Resveratrol taken together has shown this to be beneficial. Combining NMN with Resveratrol increases the NAD+ levels in the heart and muscle tissue. Professor David Sinclair, an anti-aging expert, believes that Resveratrol works synergistically with NMN. His findings show that resveratrol is needed to activate the Sirtuin genes (which protect our DNA and epigenome), whereas NMN fuels the Sirtuins.

When is the best time to take NMN and Resveratrol?

For best results, younger people should take NMN six hours after waking up, whereas older people can take NMN first thing in the morning to maximise it's energizing effects.

Pro tip Resveratrol has relatively low bioavailability, and part of NMN might be destroyed by stomach acid when consumed, thus it is important to take the bioavailable forms of both supplements. When choosing the best NMN supplement, take into account the purity, manufacturing standards, and bioavailability to reap the best benefits of this anti-aging supplement.